While FDM, SLA, two-photon laser, drop on demand are some of the most popular biofabrication methods, this “From Academia” issue includes three less well know but trending methods that could be complementary or alternative to the typical 3D bioprinting process. The first article introduces a biofabrication method using ultrasound waves, also known as sonolithography. This gentle method can rapidly generate 2D cell patterns for a variety of materials, as well as act as a complementary technique to additive manufacturing where surface patterning combined with layer‐by‐layer fabrication can facilitate the generation of structures with more internal complexity. However, this method does not allow selectively targeting and manipulating individual cells. The second article addresses exactly that problem with a single cell bioprinting method using short laser pulses, which allow for the precise and efficient selection and positioning of individual mammalian cells, as well as transferring of specific cell/cells to a target surface with precision and high cell viability. The third publication was written in 2017, but we are anticipating the author’s upcoming paper in a few weeks. This paper reviews the principles behind a fabrication technique called melt eletrowriting (or electrostatic writing) and also compares it to an adjacent technique called eletrospinning. The author also lists potential biomedical applications of this technique.
“From Academia” features recent, relevant, close to commercialization academic publications. Subjects include but not limited to healthcare 3D printing, 3D bioprinting, and related emerging technologies.
Email: Rance Tino (firstname.lastname@example.org) if you want to share relevant academic publications with us.
– Authored by Jenna M. Shapiro Bruce W. Drinkwater Adam W. Perriman Mike Fraser. Advanced Materials Technologies. December 2 2020
Acoustic fields are increasingly being used in material handling applications for gentle, noncontact manipulation of particles in fluids. Sonolithography is based on the application of acoustic radiation forces arising from the interference of ultrasonic standing waves to direct airborne particle/droplet accumulation in defined spatial regions. This approach enables reliable and repeatable patterning of materials onto a substrate to provide spatially localized topographical or biochemical cues, structural features, or other functionalities that are relevant to biofabrication and tissue engineering applications.
The technique capitalizes on inexpensive, commercially available transducers and electronics. Sonolithography is capable of rapidly patterning micrometer to millimeter scale materials onto a wide variety of substrates over a macroscale (cm2) surface area and can be used for both indirect and direct cell patterning.
– Authored by Jun Zhang Patrick Byers Amelie Erben Christine Frank Levin Schulte‐Spechtel Michael Heymann Denitsa Docheva Heinz P. Huber Stefanie Sudhop Hauke Clausen‐Schaumann. Advanced Functional Materials. March 26 2021
Tissue engineering requires the precise positioning of mammalian cells and biomaterials on substrate surfaces or in preprocessed scaffolds.
Although the development of 2D and 3D bioprinting technologies has made substantial progress in recent years, precise, cell‐friendly, easy to use, and fast technologies for selecting and positioning mammalian cells with single-cell precision are still in need.
A new laser‐based bioprinting approach is therefore presented, which allows the selection of individual cells from complex cell mixtures based on morphology or fluorescence and their transfer onto a 2D target substrate or a preprocessed 3D scaffold with single-cell precision and high cell viability (93–99% cell survival, depending on cell type and substrate).
In addition to precise cell positioning, this approach can also be used for the generation of 3D structures by transferring and depositing multiple hydrogel droplets. By further automating and combining this approach with other 3D printing technologies, such as two‐photon stereolithography, it has a high potential of becoming a fast and versatile technology for the 2D and 3D bioprinting of mammalian cells with single-cell resolution.
Keywords: single cell printing, single cell sorting, 3D bioprinting, three-dimensional printing, tissue engineering
– Authored by Paul D. Dalton. Current Opinion in Biomedical Engineering. June 2017
The recent development of electrostatic writing (electrowriting) with molten jets provides an opportunity to tackle some significant challenges within tissue engineering.
The process uses an applied voltage to generate a stable fluid jet with a predictable path that is continuously deposited onto a collector. The fiber diameter is variable during the process and is applicable to polymers with a history of clinical use.
Melt electrowriting, therefore, has the potential for clinical translation if the biological efficacy of the implant can be improved over existing gold standards. It provides a unique opportunity for laboratories to perform low-cost, high-resolution, additive manufacturing research that is well-positioned for clinical translation, using existing regulatory frameworks.
Keywords: Biomaterials, Electrohydrodynamic, Scaffold, 3D printing